Syntenin 1 Antibody (19C1) - BSA Free

Syntenin 1 Specificity is Shown by Immunocytochemistry in Knockdown Cell Line.

HAP1 parental cell line WT and Syntenin 1 HAP1 KD cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Syntenin 1 Antibody (19C1) (Catalog # NBP3-26333) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KD cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KD cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1/100. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Detection of Syntenin 1 by Immunoprecipitation.

HAP1 cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of Syntenin 1 Antibody (19C1) (Catalog # NBP3-26333) pre-coupled to Dynabeads Protein A. Immunoprecipitated Syntenin 1 was detected in Western Blot with a rabbit anti-Syntenin 1 antibody used at 1/500. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Detection of Syntenin 1 by Immunoprecipitation.

Culture medium from HAP1 cell line culture were prepared and immunoprecipitation was performed using 2.0 µg of Syntenin 1 Antibody (19C1) (Catalog # NBP3-26333) pre-coupled to Dynabeads Protein A. Immunoprecipitated Syntenin 1 was detected in Western Blot with a rabbit anti-Syntenin 1 antibody used at 1/1000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).