Synaptophysin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25170
![Western Blot: Synaptophysin AntibodyBSA Free [NBP2-25170]](https://resources.bio-techne.com/images/products/Synaptophysin-Antibody-Western-Blot-NBP2-25170-img0001.jpg "Western Blot: Synaptophysin AntibodyBSA Free [NBP2-25170]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Primate
Cited:
Human, Mouse, Rat
Predicted:
Bovine (96%), Rat (95%), Squirrel (97%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an C-terminal portion of the human Synaptophysin protein (between residues 230-313) [UniProt# P08247]
Localization
Golgi, cytoplasmic and membrane
Marker
Pre-synaptic marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Synaptophysin Antibody - BSA Free
Western Blot: Synaptophysin AntibodyBSA Free [NBP2-25170]
Western Blot: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in (A) human brain lysate and (B) mouse brain lysate.![Immunocytochemistry/ Immunofluorescence: Synaptophysin Antibody - BSA Free [NBP2-25170]](https://resources.bio-techne.com/images/products/Synaptophysin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25170-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Synaptophysin Antibody - BSA Free [NBP2-25170]")
Immunocytochemistry/ Immunofluorescence: Synaptophysin Antibody - BSA Free [NBP2-25170]
Immunocytochemistry/Immunofluorescence: Synaptophysin Antibody [NBP2-25170] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Synaptophysin at a 1:50 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry: Synaptophysin Antibody - BSA Free [NBP2-25170]](https://resources.bio-techne.com/images/products/Synaptophysin-Antibody-Immunohistochemistry-NBP2-25170-img0002.jpg "Immunohistochemistry: Synaptophysin Antibody - BSA Free [NBP2-25170]")
Immunohistochemistry: Synaptophysin Antibody - BSA Free [NBP2-25170]
Immunohistochemistry: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in mouse brain.Applications for Synaptophysin Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250
Immunohistochemistry
1:100
Immunohistochemistry-Frozen
reported in scientific literature (PMID 31117227)
Immunohistochemistry-Paraffin
1:100
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Synaptophysin
Alternate Names
SYP
Gene Symbol
SYP
UniProt
Additional Synaptophysin Products
Product Documents for Synaptophysin Antibody - BSA Free
Product Specific Notices for Synaptophysin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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