Substance P Antibody (NC1/34) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65219

Novus Biologicals, part of Bio-Techne
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NB100-65219
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Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Cited:

Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # NC1/34

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Substance P Antibodies »

Product Specifications

Immunogen

Substance P conjugated to bovine serum albumin

Reactivity Notes

Predicted cross-reactivities: Guinea Pig, Homarus sp., Human, Mammals, Lamprey, Bichir, Beaver, Amphibia, Bovine, Camel, Cancer sp., Calanus sp., Bullfrog, Cowbird, Crustacea, Crayfish, Chicken, Cockroach, Chinchilla, Chinchilla, Porcine, Porcineeon, Rabbit, Mudpuppy, Panilurus sp., Sheep, Rat, Spadefoot toad, Zebrafish

Specificity

NB100-65219 recognizes the COOH terminal end of substance P, a short polypeptide neurotransmitter that regulates the excitability of dorsal horn nociceptive neurons. It is known to have a role in several physiologic activities such as pain transmission, the vomiting reflex, salivary secretion and smooth muscle contraction. 5% reactivity is observed with eledoisin. It does not react with Leu or Met-enkephalin, somatostatin or beta-endorphin.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Substance P Antibody (NC1/34) - BSA Free

Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219]

Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219]

Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219] - Opioid R/OPRM1 (green) and Substance P (red) were detected in frozen mouse spinal cord and DRG tissue by fluorescent multiplex IHC. Mu Opioid R/OPRM1 Antibody (NB100-1620), used at 1:100, and Substance P Antibody (NB100-65219), used at 1:50, were applied to the tissue and simultaneously incubated at 4C overnight. Anti-Rabbit Alexa Fluor(R) 488-conjugated secondary antibody and anti-Rat NL557-conjugated secondary antibody (NL013) were applied to the tissue, and simultaneously incubated at room temperature, protected from light for 1 hour. Coverslips were applied to the tissue slide along with mounting media containing DAPI (blue) and imaged using a 4X objective lens.

Applications for Substance P Antibody (NC1/34) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:100 - 1:200

Immunohistochemistry-Paraffin

1:100 - 1:200
Application Notes
This product does not require antigen retrieval prior to staining of IHC-P sections. This antibody has been used for the localization of substance P in tissue sections by immunofluorescence and PAP staining in nervous systems of experimental animals and man. Please refer to the Cuello references listed below. It has also been successfully applied in archive post-mortem tissues of the human brain, see Mai and Pioro references. Use in Immunohistochemistry whole mount reported in scientific literature (PMID 24728885)

Reviewed Applications

Read 1 review rated 4 using NB100-65219 in the following applications:

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Substance P

Substance P (SP) is currently thought to be one of the most important neurotransmitters and neurotransmitters in the brain. It is an undecapeptide belonging to the tachykinin family, which consists of excitatory neuropeptides synthesized in neuronal and glial cells of the human central and peripheral nervous system. Substance P, Neurokinin A, Neuropeptide K, and Neuropeptide gamma are all generated by post-translation cleavage of the precursor Protachykinin-1. Substance P forms the major endogenous ligand for Neurokinin 1 Receptor. The pharmacology of Substance P has been associated with a number of neurological and psychiatric disorders, namely nociception, migraine, asthma, nausea, inflammatory bowel syndrome, urinary incontinence, anxiety and depression. It has also been linked to obesity. This antibody reacts with substance P, a bioactive peptide secreted from neurons both in the gut and central nervous system. Can be used for the localisation of Substance P in cells and tissues.

Alternate Names

Neurokinin-1, TAC1

Entrez Gene IDs

6863 (Human)

Gene Symbol

TAC1

UniProt

P20366 (Human)

Additional Substance P Products

Product Documents for Substance P Antibody (NC1/34) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Substance P Antibody (NC1/34) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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