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STAT1 [p Tyr701] Antibody (23GB4200)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50325

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NBP3-50325-20ul
NBP3-50325-100ul

Key Product Details

Species Reactivity

Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB4200

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-STAT1 (Y701) (Uniprot: P42224)

Modification

p Tyr701

Specificity

This antibody detects endogenous levels of STAT1 only when phosphorylated at Y701

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STAT1 [p Tyr701] Antibody (23GB4200)

STAT1 [p Tyr701] Antibody (23GB4200)

Immunocytochemistry/ Immunofluorescence: STAT1 [p Tyr701] Antibody (23GB4200)[NBP3-50325] -

Immunocytochemical staining of H9C2 cells with STAT1 antibody (1:1,000). Nuclei were stained blue with DAPI; STAT1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
STAT1 [p Tyr701] Antibody (23GB4200)

Flow Cytometry: STAT1 [p Tyr701] Antibody (23GB4200)[NBP3-50325] -

Flow cytometric analysis of STAT1expression in H9c2 cells using STAT1 antibody (1:2,000). Green, isotype control; red, Phospho-STAT1 (Y701).
STAT1 [p Tyr701] Antibody (23GB4200)

Western Blot: STAT1 [p Tyr701] Antibody (23GB4200)[NBP3-50325] -

Western blotting analysis using STAT1 (Y701) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for STAT1 [p Tyr701] Antibody (23GB4200)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT1

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1a and the 84 kDa STAT1b which are encoded by the same gene with splicing variant.

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Gene Symbol

STAT1

Additional STAT1 Products

Product Documents for STAT1 [p Tyr701] Antibody (23GB4200)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for STAT1 [p Tyr701] Antibody (23GB4200)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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