Src [p Tyr527] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13245

Novus Biologicals, part of Bio-Techne
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NBP3-13245
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%), Rhesus Macaque (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Src Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human Src (phospho Tyr529). The exact sequence is proprietary. The Tyr529 of human Src is equivalent to Tyr527 of chicken Src.

Modification

p Tyr527

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Src [p Tyr527] Antibody

Western Blot: Src [p Tyr527] Antibody [NBP3-13245]

Western Blot: Src [p Tyr527] Antibody [NBP3-13245]

Western Blot: Src [p Tyr527] Antibody [NBP3-13245] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (20 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (NBP3-13245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Src [p Tyr527] Antibody [NBP3-13245]

Western Blot: Src [p Tyr527] Antibody [NBP3-13245]

Western Blot: Src [p Tyr527] Antibody [NBP3-13245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr529) antibody (NBP3-13245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Src [p Tyr527] Antibody [NBP3-13245]

Western Blot: Src [p Tyr527] Antibody [NBP3-13245]

Western Blot: Src [p Tyr527] Antibody [NBP3-13245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (NBP3-13245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for Src [p Tyr527] Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Src

Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).

Long Name

v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog

Alternate Names

ASV, c-Src, RSVgp4

Gene Symbol

SRC

Additional Src Products

Product Documents for Src [p Tyr527] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Src [p Tyr527] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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