SP1 Antibody (23GB4175)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50324

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NBP3-50324-100ul
NBP3-50324-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB4175

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SP1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human SP1 (Uniprot: P08047)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SP1 Antibody (23GB4175)

SP1 Antibody (23GB4175)

Immunocytochemistry/ Immunofluorescence: SP1 Antibody (23GB4175)[NBP3-50324] -

Immunocytochemical staining of Hela cells with SP1 antibody (1:1,000). Nuclei were stained blue with DAPI; SP1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
SP1 Antibody (23GB4175)

Flow Cytometry: SP1 Antibody (23GB4175)[NBP3-50324] -

Flow cytometric analysis of SP1expression in HeLa cells using SP1 antibody (1:2,000). Green, isotype control; red, SP1.
SP1 Antibody (23GB4175)

Western Blot: SP1 Antibody (23GB4175)[NBP3-50324] -

Western blotting analysis using SP1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for SP1 Antibody (23GB4175)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SP1

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Alternate Names

Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1

Gene Symbol

SP1

Additional SP1 Products

Product Documents for SP1 Antibody (23GB4175)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SP1 Antibody (23GB4175)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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