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SOX11 Antibody (CL0142)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52441

Recombinant Monoclonal Antibody
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NBP3-52441

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # CL0142

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P35716, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SOX11 Antibody (CL0142)

SOX11 Antibody (CL0142) Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP3-52448]

Immunohistochemistry-Paraffin: SATB2 Antibody (CL0320) [NBP3-52448]

Staining of RH-30 cells, showing specific staining in the nucleus and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
SOX11 Antibody (CL0142) Immunohistochemistry-Paraffin: PCM1 Antibody (CL0206) [NBP3-52444]

Immunohistochemistry-Paraffin: PCM1 Antibody (CL0206) [NBP3-52444]

Staining of eye in mouse embryo E14 retina shows moderate nuclear positivity in developing retina.
SOX11 Antibody (CL0142) Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP3-52441]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP3-52441]

Staining of human chronic lymphocytic leukemia shows no nuclear positivity in tumor cells as expected.

Applications for SOX11 Antibody (CL0142)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX11

SOX11 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis.

Long Name

Transcription Factor SRY (Sex Determining Region Y)-box 11

Alternate Names

SRY (sex determining region Y)-box 11, SRY (sex-determining region Y)-box 11, SRY-related HMG-box gene 11, transcription factor SOX-11

Gene Symbol

SOX11

Additional SOX11 Products

Product Documents for SOX11 Antibody (CL0142)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SOX11 Antibody (CL0142)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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