SOX11 Antibody (CL0142) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14782
![SOX11 Antibody (CL0142) - BSA Free Immunohistochemistry: SOX11 Antibody (CL0142) [NBP3-52441]](https://resources.bio-techne.com/images/products/nbp2-14782_-immunohistochemistry-639241519768880109.jpg "Immunohistochemistry: SOX11 Antibody (CL0142) [NBP3-52441]")
![SOX11 Antibody (CL0142) - BSA Free Western Blot: SOX11 Antibody (CL0142) [NBP3-52441]](https://resources.bio-techne.com/images/products/nbp2-14782_-western-blot-639241519767565592.jpg "Western Blot: SOX11 Antibody (CL0142) [NBP3-52441]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL0142
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P48431, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Expected to cross react based on sequence identity: Rat (82%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for SOX11 Antibody (CL0142) - BSA Free
Western Blot: SOX11 Antibody (CL0142) [NBP3-52441]
Analysis in human brain tissue.![SOX11 Antibody (CL0142) - BSA Free Immunohistochemistry: SOX11 Antibody (CL0142) [NBP3-52441]](https://resources.bio-techne.com/images/products/nbp2-14782_-immunohistochemistry-639241519769864907.jpg "Immunohistochemistry: SOX11 Antibody (CL0142) [NBP3-52441]")
Immunohistochemistry: SOX11 Antibody (CL0142) [NBP3-52441]
Staining of eye in mouse embryo E14 retina shows moderate nuclear positivity in developing retina.![SOX11 Antibody (CL0142) - BSA Free Immunohistochemistry: SOX11 Antibody (CL0142) [NBP3-52441]](https://resources.bio-techne.com/images/products/nbp2-14782_-immunohistochemistry-639241519768783557.jpg "Immunohistochemistry: SOX11 Antibody (CL0142) [NBP3-52441]")
Immunohistochemistry: SOX11 Antibody (CL0142) [NBP3-52441]
Staining of human chronic lymphocytic leukemia shows no nuclear positivity in tumor cells as expected.![SOX11 Antibody (CL0142) - BSA Free Immunohistochemistry: SOX11 Antibody (CL0142) [NBP3-52441]](https://resources.bio-techne.com/images/products/nbp2-14782_-immunohistochemistry-639241519771122792.jpg "Immunohistochemistry: SOX11 Antibody (CL0142) [NBP3-52441]")
![SOX11 Antibody (CL0142) - BSA Free Immunocytochemistry/ Immunofluorescence: SOX11 Antibody (CL0142) [NBP3-52441]](https://resources.bio-techne.com/images/products/nbp2-14782_-immunocytochemistry-immunofluorescence-639241519770236743.jpg "Immunocytochemistry/ Immunofluorescence: SOX11 Antibody (CL0142) [NBP3-52441]")
Applications for SOX11 Antibody (CL0142) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Simple Western
5 ug/ml
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp and SHSY5Y, separated by Size, antibody dilution of 5 ug/mL, apparent MW was 53 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp and SHSY5Y, separated by Size, antibody dilution of 5 ug/mL, apparent MW was 53 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SOX11
Long Name
Transcription Factor SRY (Sex Determining Region Y)-box 11
Alternate Names
SRY (sex determining region Y)-box 11, SRY (sex-determining region Y)-box 11, SRY-related HMG-box gene 11, transcription factor SOX-11
Gene Symbol
SOX11
Additional SOX11 Products
Product Documents for SOX11 Antibody (CL0142) - BSA Free
Product Specific Notices for SOX11 Antibody (CL0142) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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