SIGLECL12 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38557

Novus Biologicals, part of Bio-Techne
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NBP3-38557-20ul
NBP3-38557-100ul
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Siglec-12 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-210 of human SIGLECL12 (NP_443729.1).

Sequence:
EQKDYLLTMQKSVTVQEGLCVSVLCSFSYPQNGWTASDPVHGYWFRAGDHVSRNIPVATNNPARAVQEETRDRFHLLGDPQNKDCTLSIRDTRESDAGTYVFCVERGNMKWNYKYDQLSVNVTASQDLLSRYRLEVPESVTVQEGLCVSVPCSVLYPHYNWTASSPVYGSWFKEGADIPWDIPVATNTPSG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit SIGLECL12 Antibody - BSA Free (NBP3-38557) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SIGLECL12 Antibody - BSA Free

SIGLECL12 Antibody

Western Blot: SIGLECL12 Antibody [NBP3-38557] -

Western Blot: SIGLECL12 Antibody [NBP3-38557] - Western blot analysis of various lysates using SIGLECL12 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for SIGLECL12 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Siglec-12

Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to theimmunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialicacid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily ofSIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed bytwo C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encodedprotein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containingprotein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negativeregulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster withother SIGLEC3-like genes on 19q13.4. Alternatively spliced transcript variants encoding distinct isoforms have beendescribed for this gene. (provided by RefSeq)

Long Name

Sialic Acid Binding Ig-like Lectin 12

Alternate Names

S2V, Siglec-XII, SIGLECL1, SLG

Gene Symbol

SIGLEC12

Additional Siglec-12 Products

Product Documents for SIGLECL12 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SIGLECL12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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