Senataxin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-57542
![Knockdown Validated: Senataxin Antibody [NB100-57542]](https://resources.bio-techne.com/images/products/Senataxin-Antibody-Knockdown-Validated-NB100-57542-img0008.jpg "Western Blot: Senataxin Antibody [NB100-57542]")
![Western Blot: Senataxin Antibody [NB100-57542]](https://resources.bio-techne.com/images/products/Senataxin-Antibody-Western-Blot-NB100-57542-img0006.jpg "Western Blot: Senataxin Antibody [NB100-57542]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Independent Antibodies
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Knockdown Validated
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 875 and 925 of human senataxin using the numbering given in entry NP_055861.2 (GeneID 23064).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
303 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Senataxin Antibody - BSA Free
Western Blot: Senataxin Antibody [NB100-57542]
Western Blot: Senataxin Antibody [NB100-57542] - Whole cell lysate (5 ug) from HeLa, 293T, Jurkat, and A549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.![Immunoprecipitation: Senataxin Antibody [NB100-57542]](https://resources.bio-techne.com/images/products/Senataxin-Antibody-Immunoprecipitation-NB100-57542-img0007.jpg "Immunoprecipitation: Senataxin Antibody [NB100-57542]")
Immunoprecipitation: Senataxin Antibody [NB100-57542]
Immunoprecipitation: Senataxin Antibody [NB100-57542] - Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Senataxin antibody NB100-57542 used for IP at 3 ug per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibody NB100-57543. For blotting immunoprecipitated Senataxin, NB100-57542 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for Senataxin Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 24105744). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31533039).
Reviewed Applications
Read 2 reviews rated 3 using NB100-57542 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Senataxin
Alternate Names
ALS4recessive, non-Friedreich type 1, AOA2FLJ43459, DKFZp781B151, KIAA0625FLJ12840, senataxin, SETX
Entrez Gene IDs
23064 (Human)
Gene Symbol
SETX
UniProt
Additional Senataxin Products
Product Documents for Senataxin Antibody - BSA Free
Product Specific Notices for Senataxin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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