SASH1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-26651
![Immunohistochemistry: SASH1 Antibody [NBP1-26651]](https://resources.bio-techne.com/images/products/SASH1-Antibody-Immunohistochemistry-NBP1-26651-img0004.jpg "Immunohistochemistry: SASH1 Antibody [NBP1-26651]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot (Negative)
Cited:
Immunohistochemistry-Paraffin, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human SAM and SH3 domain containing 1 using the numbering given in entry NP_056093.3 (GeneID 23328).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SASH1 Antibody - BSA Free
Immunohistochemistry: SASH1 Antibody [NBP1-26651]
Immunohistochemistry: SASH1 Antibody [NBP1-26651] - Sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti- SASH1 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and lightchain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.![Immunoprecipitation: SASH1 Antibody [NBP1-26651]](https://resources.bio-techne.com/images/products/SASH1-Antibody-Immunoprecipitation-NBP1-26651-img0002.jpg "Immunoprecipitation: SASH1 Antibody [NBP1-26651]")
Immunoprecipitation: SASH1 Antibody [NBP1-26651]
Immunoprecipitation: SASH1 Antibody [NBP1-26651] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit used for IP at 3 mcg/mg lysate. SASH1 was also immunoprecipitated by rabbit anti- SASH1 antibody NBP1-26650, which recognizes an upstream epitope.Applications for SASH1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250-1:1000
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
3-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SASH1
Alternate Names
dJ323M4, dJ323M4.1, KIAA0790RP3-323M4.1, PEPE1, Proline-glutamate repeat-containing protein, SAM and SH3 domain containing 1,2500002E12Rik, SAM and SH3 domain-containing protein 1, SH3D6A
Entrez Gene IDs
23328 (Human)
Gene Symbol
SASH1
UniProt
Additional SASH1 Products
Product Documents for SASH1 Antibody - BSA Free
Product Specific Notices for SASH1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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