SARS-CoV-2 nsp14 Antibody (HL2577)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48700

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-48700
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2577

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SARS-CoV-2 nsp14 Antibodies »

Product Specifications

Immunogen

Recombinant fragment of SARS-CoV-2 NSP14 (COVID-19) nsp14. (Wuhan-Hu-1)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SARS-CoV-2 nsp14 Antibody (HL2577) (NBP3-48700) is a recombinant monoclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SARS-CoV-2 nsp14 Antibody (HL2577)

SARS-CoV-2 nsp14 Antibody (HL2577)

Immunocytochemistry/ Immunofluorescence: SARS-CoV-2 nsp14 Antibody (HL2577) [NBP3-48700] -

SARS-CoV-2 nsp14 antibody [HL2577] detects SARS-CoV-2 nsp14 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 nsp14 stained by SARS-CoV-2 nsp14 antibody [HL2577] (NBP3-48700) diluted at 1:500.Blue: Fluoroshield with DAPI .

Applications for SARS-CoV-2 nsp14 Antibody (HL2577)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp14

SARS-CoV-2 Nonstructural Protein 14 (NSP14) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP14 is 527 amino acids (aa) with a theoretical molecular weight of 59.8 kDa (1-3). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP14 has 95.1% sequence identity and 99.1% sequence similarity (1). Functionally, NSP14 has both 3'-5' exoribonuclease activity and N7-methyltransferase activity (1-4). The N-terminal domain of NSP14 has exoribonuclease activity (ExoN) with three conserved motifs (DE, E, and D) and belongs to the DEDD superfamily (3,4). NSP14 ExoN activity has a role in RNA proofreading during viral replication (3,4). NSP10 has been shown to interact with the exoribonuclease domain of NSP14, increasing its activity (3,4). On the other hand, the carboxyl-terminal of NSP14 has the N7-methyltransferase activity and functions in viral mRNA capping (3,4).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Romano, M., Ruggiero, A., Squeglia, F., Maga, G., & Berisio, R. (2020). A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping. Cells. https://doi.org/10.3390/cells9051267

Alternate Names

ExoN, Guanine-N7 methyltransferase, Non-structural protein 14, nsp14, Proofreading exoribonuclease

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp14 Products

Product Documents for SARS-CoV-2 nsp14 Antibody (HL2577)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 nsp14 Antibody (HL2577)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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