RGM-B Antibody [mFluor Violet 450 SE]
Novus Biologicals, part of Bio-Techne | Catalog # AF3630MFV450
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RGM-B isoform 1
Gly46-SerSer411
Accession # Q6NW40
Gly46-SerSer411
Accession # Q6NW40
Specificity
Detects human RGM-B in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse RGM-B is observed and less than 1% cross-reactivity with recombinant human (rh) RGM-A and rhRGM-C is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Applications for RGM-B Antibody [mFluor Violet 450 SE]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product AF3630. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
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Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: RGM-B
The repulsive guidance molecule (RGM) family of molecules contains three uniquely structured GPI-linked glycoproteins that participate in a variety of activities. Termed RGM-A, -B and -C, they are 40-45 kDa in size, show generally nonoverlapping fetal expression patterns, and serve as BMP-4 receptors. RGM-A and -C are plasma membrane bound, while RGM-B is generally microsomal in location. RGM-A and -B are associated with neurons, while RGM-C regulates skeletal muscle and hepatocyte function.
Long Name
Repulsive Guidance Molecule B
Alternate Names
Dragon, RGMB
Gene Symbol
RGMB
Additional RGM-B Products
Product Documents for RGM-B Antibody [mFluor Violet 450 SE]
Product Specific Notices for RGM-B Antibody [mFluor Violet 450 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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