RFP Antibody (HL2028) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25634
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Non-species specific
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2028 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Full length RFP recombinant protein
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit RFP Antibody (HL2028) - Azide and BSA Free (NBP3-25634) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RFP Antibody (HL2028) - Azide and BSA Free
Western Blot: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] -
Western Blot: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted antibodies as follows: Upper image: RFP antibody [HL2028], NBP3-25634 (1:5000) Middle image: mCherry-tagged protein antibody, NBP2-43720 Lower image: FP-tagged protein antibody, The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] -
Immunocytochemistry/Immunofluorescence: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - RFP-tag antibody detects RFP tagged protein by immunofluorescent analysis. Sample: RFP tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFP tagged protein stained by RFP-tag antibody (NBP3-25634) diluted at 500. Red: RFP tagged protein is expressed in the transfected cells. Blue: Fluoroshield with DAPI .Applications for RFP Antibody (HL2028) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RFP
Alternate Names
DSRED, GFP-like chromoprotein, Red Fluorescent Protein, RFP
Additional RFP Products
Product Documents for RFP Antibody (HL2028) - Azide and BSA Free
Product Specific Notices for RFP Antibody (HL2028) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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