Western blot shows lysates of rat spinal cord tissue and rat brain (cerebellum) tissue (negative control). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Rat µ Opioid R/OPRM1 Monoclonal Antibody (Catalog # MAB86291) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for µ Opioid R/OPRM1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Rat μ Opioid R/OPRM1 Antibody

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: Rat spinal cord tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mu Opioid R/OPRM1

The mu-type opioid receptor (MOR), also known as OPRM1, is a 60‑70 kDa variably glycosylated G protein-coupled receptor that mediates the biological effects of many alkaloid and peptide opioids including morphine. MOR is primarily expressed on neurons in the brain, spinal cord, and gastrointestinal tract as well as on immune cells. MOR activation induces analgesia, euphoria, sedation, respiratory depression, and reduced intestinal motility. Following agonist binding, MOR is phosphorylated and internalized which contributes to opioid tolerance and desensitization. OPRM1 can form heterdimers with several other 7TM GPCRs including the delta-type Opioid Receptor (DOR), Nociceptin/Orphanin Receptor (ORL1), Neurokinin 1 Receptor (NK1), Somatostatin Receptor 2 (SSTR2), Cannabinoid Receptor 1, CCR5, and the alpha2A-Adrenergic Receptor (ADRA2A). Rat MOR shares 94% and 98% aa sequence identity with human and mouse MOR, respectively.

Long Name

mu Opioid Receptor

Alternate Names

LMOR, MOP, mOpioidR, MOR, MOR1, OPRM1

Entrez Gene IDs

4988 (Human); 18390 (Mouse); 25601 (Rat)

Gene Symbol

OPRM1

UniProt

P33535

Additional mu Opioid R/OPRM1 Products

Product Documents for Rat μ Opioid R/OPRM1 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Rat μ Opioid R/OPRM1 Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

Citations
Reviews

Rat μ Opioid R/OPRM1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB86291

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat μ Opioid R/OPRM1 Antibody

Detection of Rat μ Opioid R/OPRM1 by Western Blot.