Synaptotagmin-1 was detected in perfusion fixed frozen sections of rat spinal cord using 8 µg/mL Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB43641) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Rat Synaptotagmin-1 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat spinal cord

Immunoprecipitation

Matthew, W.D. et al. (1981) J. Cell Biol. 91:257.

Western Blot

Matthew, W.D. et al. (1981) J. Cell Biol. 91:257. This application was not tested by R&D Systems.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Synaptotagmin-1

The Synaptotagmins are a family of integral membrane proteins of synaptic vesicles. Rat Synaptotagmin-1 (Accession # P21707) is a 57 kDa, 421 aa glycoprotein containing two C2 domains related to protein kinase C, and sites for palmitoylation and binding of acidic phospholipids, calcium and calmodulin. Synaptotagmin-1 participates in the process of vesicular trafficking and exocytosis by inducing local Ca2⁺-dependent buckling of the plasma membrane. Rat Synaptotagmin-1 shares 98% aa identity or greater with mouse, human and bovine Synaptotagmin-1.

Alternate Names

P65, SVP65, Synaptotagmin1, SYT1

Entrez Gene IDs

6857 (Human); 20979 (Mouse); 25716 (Rat)

Gene Symbol

SYT1

Additional Synaptotagmin-1 Products

Product Documents for Rat Synaptotagmin-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat Synaptotagmin-1 Antibody

For research use only

Citations
Reviews

Rat Synaptotagmin-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB43641

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat Synaptotagmin-1 Antibody

Synaptotagmin-1 in Rat Spinal Cord.