Rat Synaptotagmin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB43641
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Western Blot, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # ASV30
Product Specifications
Immunogen
Rat brain synaptic plasma membranes
Specificity
Detects rat Synaptotagmin‑1 in Western blots. Detects Synaptotagmin-1 from rat, mouse, human, chicken, Xenopus, and fish.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Rat Synaptotagmin-1 Antibody
Synaptotagmin-1 in Rat Spinal Cord.
Synaptotagmin-1 was detected in perfusion fixed frozen sections of rat spinal cord using 8 µg/mL Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB43641) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Rat Synaptotagmin-1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat spinal cord
Sample: Perfusion fixed frozen sections of rat spinal cord
Immunoprecipitation
Matthew, W.D. et al. (1981) J. Cell Biol. 91:257.
Western Blot
Matthew, W.D. et al. (1981) J. Cell Biol. 91:257. This application was not tested by R&D Systems.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Synaptotagmin-1
The Synaptotagmins are a family of integral membrane proteins of synaptic vesicles. Rat Synaptotagmin-1 (Accession # P21707) is a 57 kDa, 421 aa glycoprotein containing two C2 domains related to protein kinase C, and sites for palmitoylation and binding of acidic phospholipids, calcium and calmodulin. Synaptotagmin-1 participates in the process of vesicular trafficking and exocytosis by inducing local Ca2+-dependent buckling of the plasma membrane. Rat Synaptotagmin-1 shares 98% aa identity or greater with mouse, human and bovine Synaptotagmin-1.
Alternate Names
P65, SVP65, Synaptotagmin1, SYT1
Gene Symbol
SYT1
Additional Synaptotagmin-1 Products
Product Documents for Rat Synaptotagmin-1 Antibody
Product Specific Notices for Rat Synaptotagmin-1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...