Detects rat CXCL3/CINC-2 alpha/ beta in ELISAs and Western blots. In sandwich immunoassays, 27.9% cross‑reactivity with recombinant rat (rr) CINC-3 is observed and no cross-reactivity or interference with rrCINC‑1, recombinant mouse (rm) KC, rmMIP-2, or recombinant human GRO alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat CXCL3/CINC-2 alpha/ beta Antibody

Chemotaxis Induced by CXCL3/CINC‑2 alpha and Neutralization by Rat CXCL3/CINC‑2 alpha/ beta Antibody.

Recombinant Rat CXCL3/ CINC-2a (Catalog # 516-CA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL3/ CINC-2a (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Rat CXCL3/CINC-2a/ beta Monoclonal Antibody (Catalog # MAB516). The ND₅₀ is typically 0.5-2.5 µg/mL.

Rat CXCL3 / GRO gamma / CINC-2 / DCIP-1 ELISA Standard Curve

Recombinant Rat CXCL3/GRO gamma/CINC-2/DCIP-1 (Catalog # 516-CA) was serially diluted and captured by Mouse Anti-Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Monoclonal Antibody (Catalog # MAB516) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF516) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Rat CXCL3/CINC-2 alpha/ beta Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Rat CXCL3/CINC-2 alpha (Catalog # 516-CA)
Recombinant Rat CXCL3/CINC-2 beta (Catalog # 517-CB)

Neutralization

Measured by its ability to neutralize CXCL3/CINC‑2 alpha-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL in the presence of 0.05 µg/mL Recombinant Rat CXCL3/CINC‑2 alpha.

Rat CXCL3/CINC-2 alpha/ beta Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Rat CXCL3/CINC-2 alpha/ beta Biotinylated Antibody (Catalog # BAF516)
Standard: Recombinant Rat CXCL3/CINC-2 alpha Protein (Catalog # 516-CA)
Standard: Recombinant Rat CXCL3/CINC-2 beta Protein (Catalog # 517-CB)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL3/GRO gamma/CINC-2/DCIP-1

CINC-2 alpha and -2 beta are mRNA splice variant isoforms that differ by only 3 amino acids at their amino-termini. These proteins are CXC chemokines most closely related to human GROs and mouse MIP-2. They are produced by granulation tissue, activated macrophages and fibroblasts. CINC-2 biological activities are mediated by CXCR1 or CXCR2.

Alternate Names

CINC-2, Dcip1, Gm1960, GRO gamma

Entrez Gene IDs

2921 (Human); 330122 (Mouse); 171551 (Rat)

Gene Symbol

CXCL3

UniProt

Q10746

Additional CXCL3/GRO gamma/CINC-2/DCIP-1 Products

Product Documents for Rat CXCL3/CINC-2 alpha/ beta Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat CXCL3/CINC-2 alpha/ beta Antibody

For research use only

Citations
Reviews

Rat CXCL3/CINC-2 alpha/ beta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB516

Key Product Details

Species Reactivity

Validated:

Cited:

Applications