Detects rat CXCL2/GRO beta/MIP‑2/CINC‑3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross‑reactivity with recombinant rat (rr) CINC-2 alpha is observed, 15% cross-reactivity with rrCINC-2 beta, recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma is observed and 5% cross-reactivity with rrCINC-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.20 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat CXCL2/GRO beta/MIP-2/CINC-3 Antibody

Chemotaxis Induced by CXCL2/CINC-3 and Neutralization by Rat CXCL2/CINC-3 Antibody.

Recombinant Rat CXCL2/CINC-3 (Catalog # 525-C3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/CINC-3 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Rat CXCL2/CINC-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF525). The ND₅₀ is typically 0.5-2.5 µg/mL.

Rat CXCL2 / GRO beta / MIP-2 / CINC-3 ELISA Standard Curve

Recombinant Rat CXCL2/GRO beta/MIP-2/CINC-3 (Catalog # 525-C3) was serially diluted and captured by Mouse Anti-Rat CXCL2/GRO beta/MIP-2/CINC-3 Monoclonal Antibody (Catalog # MAB525) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat CXCL2/GRO beta/MIP-2/CINC-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF525) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Rat CXCL2/GRO beta/MIP-2/CINC-3 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rat CXCL2/GRO beta/MIP-2/CINC-3 (Catalog # 525-C3)

Neutralization

Measured by its ability to neutralize CXCL2/GRO beta/MIP-2/CINC-3-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL in the presence of 0.1 µg/mL Recombinant Rat CXCL2/GRO beta/MIP-2/CINC-3.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL2/GRO beta/MIP-2/CINC-3

Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines.

CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs.

References

Nakagawa, H. et al. (1994) Biochem. J. 301:545.
Huang, S. et al. (1992) Am. J. Pathol. 141:981.

Alternate Names

CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2

Entrez Gene IDs

2920 (Human); 20310 (Mouse); 114105 (Rat)

Gene Symbol

CXCL2

UniProt

P30348

Additional CXCL2/GRO beta/MIP-2/CINC-3 Products

Product Documents for Rat CXCL2/GRO beta/MIP-2/CINC-3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat CXCL2/GRO beta/MIP-2/CINC-3 Antibody

For research use only

Citations
Reviews

Rat CXCL2/GRO beta/MIP-2/CINC-3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF525

Key Product Details

Species Reactivity

Validated:

Cited:

Applications