Rat BOK Antibody
R&D Systems, part of Bio-Techne | Catalog # AF825
Key Product Details
Species Reactivity
Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
KLH-coupled rat BOK synthetic peptide
ARLLRAGLSWSAPERASPAPGC
ARLLRAGLSWSAPERASPAPGC
Specificity
Detects rat BOK in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Rat BOK Antibody
Detection of Rat BOK by Western Blot.
Western blot shows lysates ofE. colitransfected with an inducible expression vector containing rat BOK. PVDF membrane was probed with 0.8 µg/mL Rabbit Anti-Rat BOK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF825) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for BOK was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.Applications for Rat BOK Antibody
Application
Recommended Usage
Western Blot
0.8 µg/mL
Sample: E. coli transfected with an inducible expression vector containing rat BOK
Sample: E. coli transfected with an inducible expression vector containing rat BOK
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BOK
BOK (Bcl-2-related Ovarian Killer) is a 23-kDa pro-apoptotic member of the Bcl-2 family of proteins. BOK has three conserved Bcl-2 homology domains (BH1, 2, and 3) and a C-terminal transmembrane domain, but does not contain the anti-apoptotic BH4 domain. BOK interacts strongly with some anti-apoptotic Bcl-2 proteins (Mcl-1, BHRF1, and A1) and not at all with pro-apoptotic Bcl-2 proteins. The tissue distribution of BOK is restricted to reproductive tissues and it is highly expressed in ovary, testis, and uterus.
Long Name
Bcl-2-related Ovarian Killer
Alternate Names
bcl2-L-9, BCL2L9bcl-2-related ovarian killer protein, Bcl-2-like protein 9, BCL2-related ovarian killer, BOKL, hBOK, MGC4631
Gene Symbol
BOK
Additional BOK Products
Product Documents for Rat BOK Antibody
Product Specific Notices for Rat BOK Antibody
For research use only
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