Rat beta-1,3-Glucuronyltransferase 1/B3GAT1 Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB6698R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Immunocytochemistry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 882302
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat β‑1,3‑Glucuronyltransferase 1/B3GAT1
Asp75-Ile347
Accession # NP_445455
Asp75-Ile347
Accession # NP_445455
Specificity
Detects rat beta‑1,3‑Glucuronyltransferase 1/B3GAT1 in ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: beta-1,3-Glucuronyltransferase 1/B3GAT1
B3GAT1 is a key enzyme involved in human natural killer-1 (HNK-1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta1-4GlcNAc-) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK-1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta1-3Gal beta1-4GlcNAc- (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3).The HNK-1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell‑cell and cell‑substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N‑terminal cytoplasmic domain and a single‑pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).
Long Name
UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase
Alternate Names
CD57, GLCATP, GLCUATP, HNK1, LEU7
Gene Symbol
B3GAT1
UniProt
Additional beta-1,3-Glucuronyltransferase 1/B3GAT1 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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