Pyruvate Carboxylase Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55361

Novus Biologicals, part of Bio-Techne
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NBP2-55361
NBP2-55361-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Carboxylase/PC Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: FAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Pyruvate Carboxylase Antibody - BSA Free (NBP2-55361) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Pyruvate Carboxylase Antibody - BSA Free

Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361]

Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361]

Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361] - Analysis using Anti-PC antibody NBP2-55361 (A) shows similar pattern to independent antibody NBP2-33407 (B).
Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-55361]

Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-55361]

Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-55361] - Staining of human cell line MCF7 shows localization to mitochondria. Antibody staining is shown in green.
Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361]

Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361]

Western Blot: Pyruvate Carboxylase Antibody [NBP2-55361] - Analysis in human cell lines HeLa and U-251M. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Applications for Pyruvate Carboxylase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pyruvate Carboxylase/PC

PCB encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Long Name

Pyruvate Carboxylase

Alternate Names

PC, PCB

Gene Symbol

PC

Additional Pyruvate Carboxylase/PC Products

Product Documents for Pyruvate Carboxylase Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Pyruvate Carboxylase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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