PTEN [p Ser29, p Thr32] Antibody - BSA Free

Unconjugated

Polyclonal Rabbit IgG

BSA Free

Please see the vial label for concentration. If unlisted please contact technical services.

This affinity purified PTEN [p Ser29, p Thr32] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 223-237 of human PTEN protein. (Uniprot: O43460)

A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse and dog sources based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

p Ser29, p Thr32

This affinity-purified antibody is directed against the phosphorylated form of human PTEN protein at the pS29 and pT32 residues. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human PTEN pS29/pT32 protein and the antibody is specific for the phosphorylated form of the proteins. Approximately 3% reactivity is observed against the non-phosphorylated form of the immunizing peptide. A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse and dog sources based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Polyclonal

Rabbit

IgG

This affinity-purified antibody is directed against the phosphorylated form of human PTEN protein at the [p Ser29] and [p Thr 32] residues. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Immunogen affinity purified

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

BSA Free

0.01% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.