PSD-95 Antibody (7E3) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12872
![Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872]](https://resources.bio-techne.com/images/products/PSD-95-Antibody-7E3-Immunocytochemistry-Immunofluorescence-NBP2-12872-img0012.jpg "Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872]")
![Western Blot: PSD-95 Antibody (7E3) [NBP2-12872]](https://resources.bio-techne.com/images/products/PSD-95-Antibody-7E3-Western-Blot-NBP2-12872-img0013.jpg "Western Blot: PSD-95 Antibody (7E3) [NBP2-12872]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Microarray
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 7E3
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant rat PSD-95
Localization
Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density , Cell Projection , Axon
Specificity
Detects ~100 kDa. An additional protein of >100 kDa is also detected. Additional cross-reactive bands are detected at ~75 kDa and 50 kDa in rat and mouse samples.
Marker
post-Synaptic Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PSD-95 Antibody (7E3) - BSA Free
Western Blot: PSD-95 Antibody (7E3) [NBP2-12872]
Western Blot: PSD-95 Antibody (7E3) [NBP2-12872] - Western Blot analysis of Rat brain membrane lysate showing detection of PSD-95 protein using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:1000.![Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872]](https://resources.bio-techne.com/images/products/PSD-95-Antibody-7E3-Immunocytochemistry-Immunofluorescence-NBP2-12872-img0015.jpg "Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872]")
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872]
Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.![Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872]](https://resources.bio-techne.com/images/products/PSD-95-Antibody-7E3-Immunohistochemistry-NBP2-12872-img0014.jpg "Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872]")
Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872]
Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872] - Immunohistochemistry analysis using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice. .![Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872]](https://resources.bio-techne.com/images/products/PSD-95-Antibody-7E3-Immunohistochemistry-NBP2-12872-img0010.jpg "Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872]")
![Immunohistochemistry-Frozen: PSD-95 Antibody (7E3) [NBP2-12872]](https://resources.bio-techne.com/images/products/PSD-95-Antibody-7E3-Immunohistochemistry-Frozen-NBP2-12872-img0011.jpg "Immunohistochemistry-Frozen: PSD-95 Antibody (7E3) [NBP2-12872]")
Applications for PSD-95 Antibody (7E3) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:1000
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:1000
Application Notes
1 ug/mL was sufficient for detection of PSD-95 on 20 ug rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary. Use in IHC-Fr reported by customer review.
Reviewed Applications
Read 1 review rated 5 using NBP2-12872 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PSD-95
Long Name
Postsynaptic Density Protein 95/Disks Large Homolog 4
Alternate Names
DLG4, PSD95, SAP90
Gene Symbol
DLG4
UniProt
Additional PSD-95 Products
Product Documents for PSD-95 Antibody (7E3) - BSA Free
Product Specific Notices for PSD-95 Antibody (7E3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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