Prohibitin 2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1807
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807]](https://resources.bio-techne.com/images/products/Prohibitin-2-Antibody-Immunohistochemistry-NB100-1807-img0004.jpg "Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Orangutan (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Repressor of Estrogen Activity (Prohibitin 2) using the numbering given in entry NP_009204.1 (GeneID 11331).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Prohibitin 2 Antibody - BSA Free
Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807]
Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- REA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: Prohibitin 2 Antibody [NB100-1807]](https://resources.bio-techne.com/images/products/Prohibitin-2-Antibody-Immunoprecipitation-NB100-1807-img0001.jpg "Immunoprecipitation: Prohibitin 2 Antibody [NB100-1807]")
Immunoprecipitation: Prohibitin 2 Antibody [NB100-1807]
Immunoprecipitation: Prohibitin 2 Antibody [NB100-1807] - Detection of Human REA by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-REA antibodies NB100-1807, another REA Ab, NB100-1808, and NB100-1809 each used for IP at 3 mcg/mg lysate. Immunoprecipitated REA was blotted using BL1706 (upper blot) or BL1707 (lower blot). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lowerApplications for Prohibitin 2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000- 1:5000
Immunohistochemistry-Paraffin
1:1000- 1:5000
Immunoprecipitation
1-4 ug/mg of lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Western Blot Not recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Prohibitin 2
Alternate Names
BAP, Bap37, BCAP37, D-Prohibitin, p22, PHB2, PNAS-141, REA
Entrez Gene IDs
11331 (Human)
Gene Symbol
PHB2
UniProt
Additional Prohibitin 2 Products
Product Documents for Prohibitin 2 Antibody - BSA Free
Product Specific Notices for Prohibitin 2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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