I'm planning to use PKC alpha Antibody (MC5) (NB600-201) for my next research, can you provide me with the protocol? I can't access the two publications stated on your website for the protocol.

We do not have any protocols specific for this particular antibody, but we do have many general technique-specific protocols. These protocols are good starting points, but we do recommend that the methods are optimized by the end user, because they will depend to some extent on the sample/reagents being used.

I'm planning to use PKC alpha Antibody (MC5) (NB600-201) for my next research. I'd like to know how many antibody kit I'll need for my sample (approx. 90 specimens).

Unfortunately, we have neither an ELISA kit for PKC alpha nor a PKC alpha extraction kit. The PKC alpha antibodies we supply are simply the primary antibodies, with no other reagents. However, we do also sell secondary antibodies, some of which are already conjugated, and antibody labeling kits. I'm afraid all I can suggest is that you consult the literature for more information on your application.

In our data base it is stated as PKC pan, on your data sheet is stated PKC alpha. Could you please confirm, if it is really PKC alpha, because from other suppliers this clone is stated for detecting other isoforms from PKC as well and in previous product lists from Novus, it is stated as "pan" as well. It would be very important, because the customer needs a product, which detects PKC pan and not only alpha.

According to our licensing agreement for this clone, it appears to be a pan PKC antibody. It is most strongly associated with PKC alpha, but testing indicates that it detects PKC beta and gamma as well.

We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.

We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

PKC alpha Antibody (MC5) - BSA Free

Name: PKC alpha Antibody (MC5) - BSA Free
Brand: Novus Biologicals
SKU: NB600-201
Rating: 5 (1 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-201

(1)

Citations (35)

Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB600-201AF350

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Alexa Fluor 405

NB600-201AF405

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Alexa Fluor 488

NB600-201AF488

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Alexa Fluor 532

NB600-201AF532

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Alexa Fluor 594

NB600-201AF594

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Alexa Fluor 647

NB600-201AF647

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Alexa Fluor 700

NB600-201AF700

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Alexa Fluor 750

NB600-201AF750

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Alexa Fluor Plus 405

NB600-201AFP405

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Alexa Fluor Plus 488

NB600-201AFP488

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Alexa Fluor Plus 555

NB600-201AFP555

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Alexa Fluor Plus 594

NB600-201AFP594

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Alexa Fluor Plus 647

NB600-201AFP647

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Alexa Fluor Plus 680

NB600-201AFP680

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Allophycocyanin

NB600-201APC

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Allophycocyanin/Cy7

NB600-201APCCY7

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Biotin

NB600-201B

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CoraFluor 1

NB600-201CL1

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DyLight 350

NB600-201UV

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DyLight 405

NB600-201V

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DyLight 488

NB600-201G

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DyLight 550

NB600-201R

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DyLight 594

NB600-201DL594

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DyLight 650

NB600-201C

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DyLight 680

NB600-201FR

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DyLight 755

NB600-201IR

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FITC

NB600-201F

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HRP

NB600-201H

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Janelia Fluor 525

NB600-201JF525

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Janelia Fluor 549

NB600-201JF549

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Janelia Fluor 646

NB600-201JF646

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Janelia Fluor 669

NB600-201JF669

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mFluor Violet 450 SE

NB600-201MFV450

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mFluor Violet 500 SE

NB600-201MFV500

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mFluor Violet 610 SE

NB600-201MFV610

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NB600-201PE

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PE/Atto594

NB600-201PEATT594

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PE/Cy5.5

NB600-201PECY55

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PE/Cy7

NB600-201PECY7

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PerCP

NB600-201PCP

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Forumulation

Catalog #

Azide and BSA Free

NBP2-80911

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100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Baboon, Bovine, Canine, Chicken, Fish, Monkey, Rabbit, Sheep, Zebrafish

Cited:

Human, Mouse, Rat, Avian - Chicken, Fish - Danio rerio (Zebrafish), Primate - Macaca mulatta (Rhesus Macaque), Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Radioimmunoassay

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # MC5

Format

BSA Free

Concentration

1.0 mg/ml

View all PKC alpha Antibodies »

Product Specifications

Immunogen

Purified bovine brain Protein Kinase C

Reactivity Notes

Use in Monkey reported in scientific literature (PMID:35024589) Use in Baboon reported in scientific literature (PMID:34314739) Zebrafish reactivity reported in scientific literature (PMID: 31273575)..

Specificity

This antibody is capable of detecting PKC beta as well.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PKC alpha Antibody (MC5) - BSA Free

Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201]

Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha (MC5) antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201]

Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - PKC alpha detected in muse prostate tissue using PKC alpha Antibody (MC5). IHC-P image submitted by a verified customer review.

Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201]

Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201] - Detection of PKC alpha in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- PKC alpha Monoclonal Antibody (Catalog # NB600-201, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201]

Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201] - IHC staining of PKC alpha in mouse brain using DAB with hematoxylin counterstain.

Western Blot: PKC alpha Antibody (MC5) - BSA Free [NB600-201] -

Expression of proteins downstream of PKM2 after SAH. A–D Western blotting and quantitative analysis of p-PKC, PKC, p-ERK and ERK. The following four kinds of mice were used for the in vivo study: WT sham mice and WT mice 24 h after SAH; PKM2CKI/CKI and Aldh1| 1-creERT2 mice 24 h after SAH; and WT mice 24 h after SAH. The cell grouping method was the same as that described in Sect. 3.6 (n = 3 per group); one-way ANOVA, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 Image collected and cropped by CiteAb from the following open publication (https://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-024…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PKC alpha Antibody (MC5) - BSA Free [NB600-201] -

The effect of DNMTs on DNA methylation of PRKCA in human cardiomyocyte cells. (a) the mRNA and (b) protein of PRKCA in AC16 cells induced by TNF-alpha, DNMTs RNA interference and 5-azacytidine. (c) the mRNA and (d) protein of PRKCA in RL-14 cells induced by TNF-alpha, DNMTs RNA interference and 5-azacytidine. DNA methylation on 5ʹ terminal of PRKCA (promoter, first exon and first intron) in (e) AC16 and (f) RL-14 cells induced by TNF-alpha, DNMTs RNA interference and 5-azacytidine. the given data from triplicate experiments was processed as mean +/- standard error and compared by student’s t-test. No. 1 to 6 indicate six comparison with control group. ‘FC’: fold change; ‘si’: siRNA; ‘AZA’: 5-azacytidine Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34482802), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PKC alpha Antibody (MC5) - BSA Free [NB600-201] -

DNA methylation of PRKCA in mitral valve of RHD. (a) the overview of CpG loci distribution within human PRKCA gene. (b) DNA methylation on 5ʹ terminal of PRKCA (promoter, first exon and first intron) in mitral valve of RHD by MeDIP-qPCR assay. (c) PRKCA transcription levels in mitral valve of RHD by qPCR assay. (d) PRKCA protein levels in mitral valve of RHD by WB assay. (e) PRKCA protein levels calculated by gray intensity analysis. (f) the correlation between DNA methylation and PRKCA transcription by pearson correlation analysis. the given data was processed as mean +/- standard error and compared between RHD and HC groups by student’s t-test. ‘RHD’: rheumatic heart disease; ‘HC’: healthy control; ‘FC’: fold change Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34482802), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PKC alpha Antibody (MC5) - BSA Free [NB600-201] -

View 6 more images

Applications for PKC alpha Antibody (MC5) - BSA Free

Application

Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Frozen

1:200-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Immunoprecipitation

1:10-1:100

Western Blot

1:1000

Application Notes

This PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.

Reviewed Applications

Read 1 review rated 5 using NB600-201 in the following applications:

Immunohistochemistry-Paraffin (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Tris-Glycine and 0.15 M NaCl

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC alpha

Protein Kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanisms. PKC alpha belongs to the conventional PKC group, alongside Beta-I, Beta-II, and Gamma, and is activated by phosphatidylserine in a calcium dependent manner. Furthermore, PKC alpha is localized to the membrane and cytoplasm, but in some cases can translocate to the nucleus. PKC alpha helps to positively and negatively regulate cell proliferation, apoptosis, differentiation, adhesion, and migration via phosphorylation of RAF1, BCL2, CSPG4, TNNT2/CTNT, or MAPK1/3. It also exhibits positive interactions with ADAP1/CENTA1, CSPG4 and PRKCABP. PKC alpha demonstrates anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3 (PMCID: PMC1360842). Additional research has shown PKC alpha's involvement in cellular inflammation (PMCID: PMC3080812).

Long Name

Protein Kinase C alpha

Alternate Names

PKCA, PRKCA

Entrez Gene IDs

5578 (Human); 75292 (Mouse); 313834 (Rat); 282001 (Bovine)

Gene Symbol

PRKCA

UniProt

P17252, P04409 (Bovine)

Additional PKC alpha Products

Product Documents for PKC alpha Antibody (MC5) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for PKC alpha Antibody (MC5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

PKC alpha Antibody (MC5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-201

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all PKC alpha Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Specificity

Clonality

Host

Isotype

Scientific Data Images for PKC alpha Antibody (MC5) - BSA Free

Immunocytochemistry/ Immunofluorescence: PKC alpha Antibody (MC5) [NB600-201]

Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201]

Flow Cytometry: PKC alpha Antibody (MC5) [NB600-201]

Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [NB600-201]

Western Blot: PKC alpha Antibody (MC5) - BSA Free [NB600-201] -

Western Blot: PKC alpha Antibody (MC5) - BSA Free [NB600-201] -

Western Blot: PKC alpha Antibody (MC5) - BSA Free [NB600-201] -

Western Blot: PKC alpha Antibody (MC5) - BSA Free [NB600-201] -

Western Blot: PKC alpha Antibody (MC5) - BSA Free [NB600-201] -

Applications for PKC alpha Antibody (MC5) - BSA Free

ELISA

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin

Immunoprecipitation

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: PKC alpha

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional PKC alpha Products

Product Documents for PKC alpha Antibody (MC5) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for PKC alpha Antibody (MC5) - BSA Free