PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32788
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE45-17 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr197 of PKA C (pan). (Uniprot: P17612)
Modification
p Thr 197
Localization
Cytoplasm, Cell membrane, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit) (NBP3-32788) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)
![PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)](https://resources.bio-techne.com/images/products/nbp3-32788_rabbit-pka-c-pan-p-thr197-mab-je45-17-288202412461616.jpg "Western Blot: PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit) [NBP3-32788]")
Western Blot: PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit) [NBP3-32788]
Western Blot: PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit) [NBP3-32788] - Western blot analysis of PKA C (pan) on different lysates with Rabbit anti-PKA C (pan) antibody (NBP3-32788) at 1/1,000 dilution.Lane 1: NIH/3T3 cell lysate
Lane 2: C6 cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32788) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PKA C (pan)
The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription. Catalytic PKA subunit isoforms, Cα, Cβ and, in humans, Cγ, have been identified.
Long Name
cAMP-dependent Protein Kinase Catalytic Subunit
Alternate Names
CAFD1, PKACA, PPNAD4
Gene Symbol
PRKACA
Additional PKA C (pan) Products
Product Documents for PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)
Product Specific Notices for PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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