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PKA C beta Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-46894

Novus Biologicals, part of Bio-Techne
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NBP3-46894

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

protein kinase, cAMP-dependent, catalytic, beta. (Uniprot# P22694)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit PKA C beta Antibody - BSA Free (NBP3-46894) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PKA C beta Antibody - BSA Free

PKA C beta Antibody - BSA Free

Western Blot: PKA C beta Antibody - BSA Free [NBP3-46894] -

Western Blot: PKA C beta Antibody - BSA Free [NBP3-46894] - HT-1080 cells were subjected to SDS PAGE followed by western blot with (PKA C beta antibody) at dilution of 1:1000
PKA C beta Antibody - BSA Free

Immunohistochemistry: PKA C beta Antibody - BSA Free [NBP3-46894] -

Immunohistochemistry: PKA C beta Antibody - BSA Free [NBP3-46894] - Immunohistochemistry of paraffin-embedded human medulloblastoma using (PKA C beta antibody) at dilution of 1:50
PKA C beta Antibody - BSA Free

Immunoprecipitation: PKA C beta Antibody - BSA Free [NBP3-46894] -

Immunoprecipitation: PKA C beta Antibody - BSA Free [NBP3-46894] - IP Result of anti-PKA C beta (IP:, 4ug; Detection: 1:500) with mouse brain tissue lysate 2640ug.

Applications for PKA C beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:20-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
Theoretical Molecular Weight is 46-55 kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKA C beta

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Calpha, Cbeta and Cgamma, that each represent specific gene products. Calpha and Cbeta are closely related (93% amino acid sequence similarity), whereas Cgamma displays 83% and 79% similarity to Calpha and Cbeta, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit beta

Alternate Names

cAPKbeta, PRKACB

Gene Symbol

PRKACB

Additional PKA C beta Products

Product Documents for PKA C beta Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PKA C beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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