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PKA C alpha Antibody (23GB1030)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52025

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NBP3-52025-100ul
NBP3-52025-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1030

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human PKA C alpha (Uniprot: P17612)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKA C alpha Antibody (23GB1030)

PKA C alpha Antibody (23GB1030)

Immunocytochemistry/ Immunofluorescence: PKA C alpha Antibody (23GB1030)[NBP3-52025] -

Immunocytochemical staining of HeLa cells with PKA C alpha antibody (1:1,000). Nuclei were stained blue with DAPI; PKA C alpha was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
PKA C alpha Antibody (23GB1030)

Flow Cytometry: PKA C alpha Antibody (23GB1030)[NBP3-52025] -

Flow cytometric analysis of PKA C alphaexpression in C2C12 cells using PKA C alpha antibody (1:2,000). Green, isotype control; red, cAMP Protein Kinase Catalytic Subunit.

Applications for PKA C alpha Antibody (23GB1030)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKA C alpha

PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit alpha

Alternate Names

Cs, Pkaca, PKCA1, PKCD, PRKACA

Gene Symbol

PRKACA

Additional PKA C alpha Products

Product Documents for PKA C alpha Antibody (23GB1030)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PKA C alpha Antibody (23GB1030)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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