PIDD Antibody (Anto-1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97595
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # Anto-1
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human PIDD death domain (aa 776-910).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PIDD Antibody (Anto-1) - BSA Free
Western Blot: PIDD Antibody (Anto-1)BSA Free [NBP1-97595]
Western Blot: LRDD Antibody (Anto-1) [NBP1-97595] - Analysis of endogenous and overexpressed human PIDD using MAb to PIDD (Anto-1). Method: Cell extracts (1x10^5) from HEK 293T wild type (lane 1) or transfected with a plasmid coding for human PIDD (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to PIDD (Anto-1) at a 1:500 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system. Note that two major forms of PIDD are detected, representing full length and cleaved proteins, migrating at approximatively 90kDa and 50kDa, respectively.Immunohistochemistry-Paraffin: PIDD Antibody (Anto-1) - BSA Free [NBP1-97595]
Immunohistochemistry-Paraffin: LRDD Antibody (Anto-1) [NBP1-97595] - Staining of endogenous human PIDD in different human tissues (paraffin sections) using MAb to PIDD (Anto-1). Method: Different human normal (A: Bronchus; B: Kidney; C: Lung) or cancer (D: Breast; E: Ovarian; F: Colo-rectal) tissues were stained with MAb to PIDD (Anto-1) by standard immunohistochemistry.Applications for PIDD Antibody (Anto-1) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500
Application Notes
Detects bands of ~90kDa (full length protein) and ~50kDa (cleaved protein) by Western blot.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PIDD
Long Name
p53-Induced Protein With A Death Domain
Alternate Names
LRDD
Gene Symbol
PIDD1
Additional PIDD Products
Product Documents for PIDD Antibody (Anto-1) - BSA Free
Product Specific Notices for PIDD Antibody (Anto-1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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