phycoerythrin Antibody (PE-DLF) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00146
![Flow Cytometry: phycoerythrin Antibody (PE-DLF) - BSA Free [NBP2-00146]](https://resources.bio-techne.com/images/products/phycoerythrin-Antibody-PE-DLF-Flow-Cytometry-NBP2-00146-img0003.jpg "Flow Cytometry: phycoerythrin Antibody (PE-DLF) - BSA Free [NBP2-00146]")
Key Product Details
Species Reactivity
Non-species specific
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PE-DLF
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody is Phycoerythrin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Novus Biologicals Mouse phycoerythrin Antibody (PE-DLF) - BSA Free (NBP2-00146) is a monoclonal antibody validated for use in IHC, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for phycoerythrin Antibody (PE-DLF) - BSA Free
Flow Cytometry: phycoerythrin Antibody (PE-DLF) - BSA Free [NBP2-00146]
Flow Cytometry: phycoerythrin Antibody (PE-DLF) [NBP2-00146] - Analysis using the Biotin conjugate of NBP2-00146. Staining of C57BL/6 splenocytes with Anti Human/Mouse CD45R (B220) PE and 0.06 ug of Mouse IgG1 kappa Isotype Control Biotin (left) or 0.06 ug of Anti-Phycoerythrin Biotin (right) followed by Streptavidin APC.![Flow Cytometry: phycoerythrin Antibody (PE-DLF) - BSA Free [NBP2-00146]](https://resources.bio-techne.com/images/products/phycoerythrin-Antibody-PE-DLF-Flow-Cytometry-NBP2-00146-img0001.jpg "Flow Cytometry: phycoerythrin Antibody (PE-DLF) - BSA Free [NBP2-00146]")
Flow Cytometry: phycoerythrin Antibody (PE-DLF) - BSA Free [NBP2-00146]
Flow Cytometry: phycoerythrin Antibody (PE-DLF) [NBP2-00146] - Staining of normal human peripheral blood cells with Rat Anti-Human/Mouse CD44 PE , followed by 0.125 ug of Mouse IgG1 K Isotype Control Purified (left) or 0.125 ug of Anti-Phycoerythrin Purified (right), followed by detection with Anti-Mouse IgG FITC. Total viable cells were used for analysis.Applications for phycoerythrin Antibody (PE-DLF) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Application Notes
This PE-DLF antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: phycoerythrin
Alternate Names
PE, R-PE
Additional phycoerythrin Products
Product Documents for phycoerythrin Antibody (PE-DLF) - BSA Free
Product Specific Notices for phycoerythrin Antibody (PE-DLF) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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