Detects endogenous proteins containing phosphorylated tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. Does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Phospho-Tyrosine HRP-conjugated Antibody

Detection of Phospho-Tyrosine by Western Blot.

Phospho-PDGF R beta ELISA Using HAM1676 as Detection Antibody.

Applications for Phospho-Tyrosine HRP-conjugated Antibody

Application

Recommended Usage

ELISA

Western Blot

Formulation, Preparation, and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers and preservatives.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Phospho-Tyrosine

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine HRP-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Phospho-Tyrosine HRP-conjugated Antibody

For research use only

Citations
Reviews

Phospho-Tyrosine HRP-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # HAM1676

Key Product Details

Species Reactivity

Validated:

Cited:

Applications