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PHLPP Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-47157

Novus Biologicals, part of Bio-Techne
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NBP3-47157

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PH domain and leucine rich repeat protein phosphatase. (Uniprot# O60346)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PHLPP Antibody - BSA Free (NBP3-47157) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PHLPP Antibody - BSA Free

PHLPP Antibody - BSA Free

Immunohistochemistry: PHLPP Antibody - BSA Free [NBP3-47157] -

Immunohistochemistry: PHLPP Antibody - BSA Free [NBP3-47157] - Immunohistochemistry of paraffin-embedded human liver slide using (PHLPP Antibody) at dilution of 1:50
PHLPP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PHLPP Antibody - BSA Free [NBP3-47157] -

Immunocytochemistry/ Immunofluorescence: PHLPP Antibody - BSA Free [NBP3-47157] - Immunofluorescent analysis of HepG2 cells, using PHLPP antibody at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
PHLPP Antibody - BSA Free

Immunoprecipitation: PHLPP Antibody - BSA Free [NBP3-47157] -

Immunoprecipitation: PHLPP Antibody - BSA Free [NBP3-47157] - IP Result of anti-PHLPP (IP: , 4ug; Detection: 1:1000) with mouse brain tissue lysate 5000ug.

Applications for PHLPP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:20-1:200

Immunoprecipitation

1:500-1:2000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PHLPP

PHLPP (PH domain leucine-rich repeat-containing protein phosphatase) is responsible for the dephosphorylation and subsequent inactivation of Akt. Akt is a signaling protein critical to the balance of cell survival and apoptosis. Dephosphorylation of Akt by PHLPP has been demonstrated to trigger apoptosis and suppress tumor cell growth. PHLPP functions similarly to its relative PHLPPL, also known as PHLPPL2. Recent studies show that PHLPP and PHLPPL selectively terminate Akt-signaling by targeting specific Akt isoforms. Alternate names for PHLPP include pleckstrin homology domain-containing family E protein 1, suprachiasmatic nucleus circadian oscillatory protein, hSCOP, PHLPP1, KIAA0606, PLEKHE1, and SCOP.

Alternate Names

EC 3.1.3.16, hSCOP, KIAA0606, MGC161555, PH domain and leucine rich repeat protein phosphatase, PH domain and leucine rich repeat protein phosphatase 1, PH domain leucine-rich repeat-containing protein phosphatase 1, PHLPP, pleckstrin homology domain containing, family E (with leucine rich repeats)member 1, Pleckstrin homology domain-containing family E member 1, PLEKHE1, SCN circadian oscillatory protein, SCOPPH domain-containing family E member 1, Suprachiasmatic nucleus circadian oscillatory protein

Gene Symbol

PHLPP1

Additional PHLPP Products

Product Documents for PHLPP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PHLPP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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