PHC3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-77312
![Western Blot: PHC3 Antibody [NB100-77312]](https://resources.bio-techne.com/images/products/PHC3-Antibody-Western-Blot-NB100-77312-img0003.jpg "Western Blot: PHC3 Antibody [NB100-77312]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human polyhomeotic like 3 using the numbering given in entry AAI31773.1 (GeneID 80012).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PHC3 Antibody - BSA Free (NB100-77312) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PHC3 Antibody - BSA Free
Western Blot: PHC3 Antibody [NB100-77312]
Western Blot: PHC3 Antibody [NB100-77312] - Detection of human PHC3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Western Blot: PHC3 Antibody [NB100-77312]](https://resources.bio-techne.com/images/products/PHC3-Antibody-Western-Blot-NB100-77312-img0002.jpg "Western Blot: PHC3 Antibody [NB100-77312]")
Western Blot: PHC3 Antibody [NB100-77312]
Western Blot: PHC3 Antibody [NB100-77312] - Detection of Human PHC3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PHC3 was also immunoprecipitated by another rabbit anti-PHC3 antibody, which recognizes a downstream epitope.![Immunoprecipitation: PHC3 Antibody [NB100-77312]](https://resources.bio-techne.com/images/products/PHC3-Antibody-NB100-77312-img0004.jpg "Immunoprecipitation: PHC3 Antibody [NB100-77312]")
Immunoprecipitation: PHC3 Antibody [NB100-77312]
Immunoprecipitation: PHC3 Antibody [NB100-77312] - Detection of human PHC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for IP at 3 ug per reaction. PHC3 was also immunoprecipitated by rabbit anti-PHC3 antibody NB100-77313. For blotting immunoprecipitated PHC3, NB100-77312 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for PHC3 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PHC3
Alternate Names
DKFZp313K1221, early development regulator 3, Early development regulatory protein 3, EDR3FLJ12729, FLJ12967, Homolog of polyhomeotic 3, HPH3, MGC88144, PH3, polyhomeotic homolog 3 (Drosophila), polyhomeotic like 3, polyhomeotic like 3 (Drosophila), polyhomeotic-like protein 3
Entrez Gene IDs
80012 (Human)
Gene Symbol
PHC3
UniProt
Additional PHC3 Products
Product Documents for PHC3 Antibody - BSA Free
Product Specific Notices for PHC3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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