Phantom Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-658

Novus Biologicals, part of Bio-Techne
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NB300-658
NB300-658-0.025mg
NB300-658-0.05mg
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Key Product Details

Species Reactivity

Drosophila

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.75 mg/ml

View all Phantom Antibodies »

Product Specifications

Immunogen

Synthetic peptide for Saxophone antibodies are (WIT-101AP XXXXXXXXXXXXXXXXXX and; WIT-112APXXXXXXXXXXXXXXXXXXXXXXX; All peptides were amidated for conjugation.

Reactivity Notes

Cross-reacts with Drosophilia melanogaster.Not yet tested in other species.

Specificity

Phantom

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Phantom Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Immunocytochemistry, Immunoprecipitation and Western Blot: Optimal dilutions and concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

0.75 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phantom

Fruit fly (Drosophila melanogaster) ovaries contains two set of germline stem cells surrounded by a group of highly differentiated somatic cells that express genes for two phenotypes (hedgehog & wingless). The TGF beta super family member, decpentaplegic (dpp) or its homologue BMP2/4 is specifically required for maintenance and promote its cell division in the female germline (1, 2). The Signaling by TGF beta-related factors requires ligand-induced association between type I and type II transmembrane receptors that have endogenous serine/threonine kinases activity. In Drosophila, the saxophone (sax) and thick veins (tkv) genes encode type I receptors that mediate signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta-type factors. Where as Punt and Wishful Thinking (wit) are type II receptors. Over expression or mutation in dpp suppress germline stem cell differentiation. Dpp actions are mediated by its receptor Saxophone. The Saxophone gene is expressed ubiquitously. The Saxophone gene also gives two products Brk43E and Berk25 and both gene products inter acts with TGF super family peptide ligands Dpp. Mutations that completely abolish Saxophone activity causes phenotype that are similar to partial or complete loss of activity of the dpp ligand. The saxophone products are also have serine/threonine kinase activity responsible for phsosphorylation and activation of the ligands including pMAD, Screw (Scw), and short gastrulation protein (Sog). The mutant larvae showed small synapses, defective evoke potentials and slower vesicular release and changes in the ultra-structural architect (3). Mutations in the Phantom gene disrupts the neuronal remodeling in mushroom bodies (MBs) in fruit fly (4). The Anti-Phantom-selective antibodies were generated against conserved sequences near the N-terminus and the C-terminal end of the protein that are unique to D. melanogaster Phantom protein. The Anti-Phantom-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. Anti-Phantom-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses.

Alternate Names

Exo, Exocephalon

Additional Phantom Products

Product Documents for Phantom Antibody

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Product Specific Notices for Phantom Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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