PGK1 Antibody (24GB2935)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51612
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG2a Kappa Clone # 24GB2935
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein of human PGK1 (Uniprot: P00558)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG2a Kappa
Scientific Data Images for PGK1 Antibody (24GB2935)
Immunocytochemistry/ Immunofluorescence: PGK1 Antibody (24GB2935)[NBP3-51612] -
PGK1 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: Oxoglutarate receptor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Oxoglutarate receptor 1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
Immunocytochemistry/ Immunofluorescence: PGK1 Antibody (24GB2935)[NBP3-51612] -
Immunocytochemical staining of HepG2 cells with PGK1 antibody(1:1,000) . Nuclei were stained blue with DAPI; Phosphoglycerate kinase 1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Western Blot: PGK1 Antibody (24GB2935)[NBP3-51612] -
Western blotting analysis using PGK1 antibody (1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.Applications for PGK1 Antibody (24GB2935)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PGK1
Long Name
Phosphoglycerate Kinase-1
Alternate Names
MIG10, PGKA, PRP2
Gene Symbol
PGK1
Additional PGK1 Products
Product Documents for PGK1 Antibody (24GB2935)
Product Specific Notices for PGK1 Antibody (24GB2935)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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