Peroxiredoxin 6 Antibody (24GB910)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51466

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NBP3-51466-100ul
NBP3-51466-20ul
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB910

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Peroxiredoxin 6 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Peroxiredoxin 6 (Uniprot: P30041)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Peroxiredoxin 6 Antibody (24GB910)

Peroxiredoxin 6 Antibody (24GB910)

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 6 Antibody (24GB910)[NBP3-51466] -

Peroxiredoxin 6 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: Peroxiredoxin 6 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Peroxiredoxin 6 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
Peroxiredoxin 6 Antibody (24GB910)

Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 6 Antibody (24GB910)[NBP3-51466] -

Immunocytochemical staining of HepG2 cells with Peroxiredoxin 6 antibody (1:1,000). Nuclei were stained blue with DAPI; Peroxiredoxin 6 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Peroxiredoxin 6 Antibody (24GB910)

Flow Cytometry: Peroxiredoxin 6 Antibody (24GB910)[NBP3-51466] -

Validation of Peroxiredoxin 6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Peroxiredoxin 6 antibody (1:2,000) and analyzed using BD flow cytometer.
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Applications for Peroxiredoxin 6 Antibody (24GB910)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 6

FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. CATALYTIC ACTIVITY: 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. SUBUNIT: Homotetramer. May interact with HTR2A. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Note=Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.

Alternate Names

aiPLA2, AOP2, NSGPx, PRDX6

Gene Symbol

PRDX6

Additional Peroxiredoxin 6 Products

Product Documents for Peroxiredoxin 6 Antibody (24GB910)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Peroxiredoxin 6 Antibody (24GB910)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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