PDGF R beta [p Tyr1009] Antibody (JE46-72)

Western Blot: PDGF R beta [p Tyr1009] Antibody (JE46-72) [NBP3-32785]

Western blot analysis on different lysates with NBP3-32785 at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 100ng/mL PDGF for 5 minutescell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa (Glycosylation) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32785 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Western Blot: PDGF R beta [p Tyr1009] Antibody (JE46-72) [NBP3-32785]

Western blot analysis on different lysates with NBP3-32785 at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa (Glycosylation) Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32785 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.