PCAF Antibody (23GB910)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51869

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NBP3-51869-100ul
NBP3-51869-20ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB910

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PCAF Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PCAF (Uniprot: Q92831)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCAF Antibody (23GB910)

PCAF Antibody (23GB910)

Immunocytochemistry/ Immunofluorescence: PCAF Antibody (23GB910)[NBP3-51869] -

Immunocytochemical staining of HT-1080 cells with PCAF antibody (1:1,000). Nuclei were stained blue with DAPI; PCAF was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
PCAF Antibody (23GB910)

Flow Cytometry: PCAF Antibody (23GB910)[NBP3-51869] -

Flow cytometric analysis of PCAFexpression in HT-1080 cells using PCAF antibody (1:2,000). Green, isotype control; red, PCAF.
PCAF Antibody (23GB910)

Western Blot: PCAF Antibody (23GB910)[NBP3-51869] -

Western blotting analysis using PCAF antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for PCAF Antibody (23GB910)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PCAF

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

Long Name

p300/CBP-associated Factor

Alternate Names

GCN5, KAT2B

Gene Symbol

KAT2B

Additional PCAF Products

Product Documents for PCAF Antibody (23GB910)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PCAF Antibody (23GB910)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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