PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (4E1H7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16920

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16920-100ul
NBP3-16920-20ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4E1H7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAK4+5+6 Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S474 of human PAK4+5+6 (O96013). RKSLV

Modification

p Ser 474, p Ser 560, p Ser 602

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (4E1H7)

Western Blot: PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (4E1H7) [NBP3-16920] - Western blot analysis of extracts of various cell lines, using PAK4+5+6 Rabbit mAb (NBP3-16920) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (4E1H7)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAK4+5+6

Three recently identified isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiaekinase STE20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.

Alternate Names

EC 2.7.11, EC 2.7.11.1, KIAA1142, p21 protein (Cdc42/Rac)-activated kinase 4, p21(CDKN1A)-activated kinase 4, p21-activated kinase 4, PAK-4, protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs, serine/threonine-protein kinase PAK 4

Gene Symbol

PAK4

Additional PAK4+5+6 Products

Product Documents for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (4E1H7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (4E1H7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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