p73 Antibody (7L1V7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16142
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7L1V7 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p73 (O15350). MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit p73 Antibody (7L1V7) (NBP3-16142) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for p73 Antibody (7L1V7)
![Western Blot: p73 Antibody (7L1V7) [NBP3-16142]](https://resources.bio-techne.com/images/products/p73-Antibody-7L1V7-Western-Blot-NBP3-16142-img0004.jpg "Western Blot: p73 Antibody (7L1V7) [NBP3-16142]")
Western Blot: p73 Antibody (7L1V7) [NBP3-16142]
Western Blot: p73 Antibody (7L1V7) [NBP3-16142] - Western blot analysis of extracts of various cell lines, using p73 antibody (NBP3-16142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry: p73 Antibody (7L1V7) [NBP3-16142] -
Immunohistochemistry: p73 Antibody (7L1V7) [NBP3-16142] - Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using p73 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry: p73 Antibody (7L1V7) [NBP3-16142] -
Immunohistochemistry: p73 Antibody (7L1V7) [NBP3-16142] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using p73 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.Applications for p73 Antibody (7L1V7)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: p73
Long Name
Tumor Protein p73
Alternate Names
TP73
Gene Symbol
TP73
Additional p73 Products
Product Documents for p73 Antibody (7L1V7)
Product Specific Notices for p73 Antibody (7L1V7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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