p27/Kip1 Antibody (rKIP1/1356)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49056
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rKIP1/1356 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human CDKN1B protein (Uniprot: P46527)
Localization
Cytoplasm, Endosome, Nucleus
Specificity
This antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Scientific Data Images for p27/Kip1 Antibody (rKIP1/1356)
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (rKIP1/1356) [NBP3-49056] -
Formalin-fixed, paraffin-embedded human breast carcinoma stained with p27/Kip1 Antibody (rKIP1/1356). Inset: PBS instead of primary antibody. Secondary negative control.
Western Blot: p27/Kip1 Antibody (rKIP1/1356) [NBP3-49056] -
Western blot analysis of 3T3 lysate using p27/Kip1 Antibody (rKIP1/1356).Applications for p27/Kip1 Antibody (rKIP1/1356)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: p27/Kip1
Long Name
p27 Cyclin Dependent Kinase 4 Inhibitor 1B
Alternate Names
CDKN1B, Kip1
Gene Symbol
CDKN1B
Additional p27/Kip1 Products
Product Documents for p27/Kip1 Antibody (rKIP1/1356)
Product Specific Notices for p27/Kip1 Antibody (rKIP1/1356)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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