p16INK4a/CDKN2A Antibody (GT1249) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13669

Novus Biologicals, part of Bio-Techne
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NBP3-13669
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT1249

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p16INK4a / CDKN2A Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human p16INK4a/CDKN2A. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for p16INK4a/CDKN2A Antibody (GT1249) - Azide and BSA Free

Western Blot: p16INK4a/CDKN2A Antibody (GT1249) [NBP3-13669]

Western Blot: p16INK4a/CDKN2A Antibody (GT1249) [NBP3-13669]

Western Blot: p16INK4a/CDKN2A Antibody (GT1249) [NBP3-13669] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p16INK4a/CDKN2A antibody [GT1249] (NBP3-13669) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (GT1249) [NBP3-13669]

Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (GT1249) [NBP3-13669]

Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (GT1249) [NBP3-13669] - p16INK4a/CDKN2A antibody [GT1249] detects p16INK4a/CDKN2A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast. p16INK4a/CDKN2A stained by p16INK4a/CDKN2A antibody [GT1249] (NBP3-13669) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for p16INK4a/CDKN2A Antibody (GT1249) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p16INK4a / CDKN2A

CDKN2A/p16INK4a is a gene that codes for a widely expressed protein with four isoforms, measuring 156, 105, 116, and 167 amino acids in length with weights of approximately 17, 11, 12, and 18 kDa respectively. CDKN2A/p16INK4a functions as a negative regulator of the proliferation of cells, which then allows them to interact with cyclins D and to phosphorylate the retinoblastoma protein. Current studies are being done on several diseases and disorders related to this gene including Li-Fraumeni syndrome, malignant peripheral nerve sheath tumor, blastic plasmacytoid dendritic cell, acth-secreting pituitary adenoma, recessive dystrophic epidermolysis bullosa, marginal zone b-cell lymphoma, adult astrocytic tumor, melanoma, and squamous cell carcinoma. CDKN2A/p16INK4a has also been shown to have interactions with MDM2, ZNF420, EGR1, MYCN, and CDK6 in pathways such as the cell cycle, p53 signaling, mitochondrial apoptosis, HIF-1 Alpha, CRHR, pancreatic cancer, and glioma pathways.

Long Name

p16 Cyclin Dependent Kinase 4 Inhibitor 2A

Alternate Names

ARF, CDK4I, CDKN2A, INK4a, MLM, p14ARF, p16, p19ARF

Gene Symbol

CDKN2A

Additional p16INK4a / CDKN2A Products

Product Documents for p16INK4a/CDKN2A Antibody (GT1249) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p16INK4a/CDKN2A Antibody (GT1249) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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