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Nucleoli Marker Antibody (NM95) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34695

Novus Biologicals, part of Bio-Techne
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NBP2-34695-0.1mg

Key Product Details

Species Reactivity

Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Applications

Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NM95

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Nuclei of myeloid leukemia biopsy cells

Reactivity Notes

Does not react with Mouse, Rat or Bovine.

Localization

Nucleoli

Specificity

This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Marker

Marker For Human Cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32886).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Nucleoli Marker Antibody (NM95) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Nucleoli Marker Antibody (NM95) - Azide and BSA Free [NBP2-34695]

Immunocytochemistry/ Immunofluorescence: Nucleoli Marker Antibody (NM95) - Azide and BSA Free [NBP2-34695]

Immunocytochemistry/Immunofluorescence: Nucleoli Marker Antibody (NM95) - Azide and BSA Free [NBP2-34695] - Immunofluorescence Analysis of PFA-fixed K562 cells labeling Nucleolin with followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.
Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - Azide and BSA Free [NBP2-34695]

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - Azide and BSA Free [NBP2-34695]

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - Azide and BSA Free [NBP2-34695] - Staining of human colon ca.
Flow Cytometry: Nucleoli Marker Antibody (NM95) - Azide and BSA Free [NBP2-34695]

Flow Cytometry: Nucleoli Marker Antibody (NM95) - Azide and BSA Free [NBP2-34695]

Flow Cytometry: Nucleoli Marker Antibody (NM95) - Azide and BSA Free [NBP2-34695] - Flow Cytometry of human nucleolar Ag on 293T cells Black: cells alone: Grey: isotype control: Green: AF488-labeled human Nucleolar Monoclonal antibody (NM95)

Applications for Nucleoli Marker Antibody (NM95) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5-1.0ug/ml
Application Notes
Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT.
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Nucleoli Marker

Additional Nucleoli Marker Products

Product Documents for Nucleoli Marker Antibody (NM95) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nucleoli Marker Antibody (NM95) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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