NQO-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1005

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NB100-1005
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

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Product Specifications

Immunogen

Peptide with sequence C-SIPTDNQIKARK corresponding to C-Terminus according to NP_000894.1, NP_001020604.1, NP_001020605.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22520446). Expected from sequence similarity: Canine

Specificity

This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NQO-1 Antibody

Western Blot: NQO-1 Antibody [NB100-1005]

Western Blot: NQO-1 Antibody [NB100-1005]

Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: NQO-1 Antibody [NB100-1005]

Immunocytochemistry/ Immunofluorescence: NQO-1 Antibody [NB100-1005]

Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NB100-1005] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry: NQO-1 Antibody [NB100-1005]

Immunohistochemistry: NQO-1 Antibody [NB100-1005]

Immunohistochemistry: NQO-1 Antibody [NB100-1005] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for NQO-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2.0 - 4.0 ug/ml

Immunohistochemistry-Paraffin

2.0 - 4.0 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx. 30kDa band observed in lysates of cell lines U251 and HepG2 and in Human Kidney, Lung and Duodenum lysates, and approx. 30-32kDa band in Rat Kidney, Lung and Duodenum lysates (calculated MW of 30.9kDa according to Human NP_000894.1 and Rat NP_058696.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NQO-1

NQO1 is a 2-electron reductase that detoxifies quinones derived from the oxidation of phenolic metabolites of benzene. Individuals homozygous for the T/T form of the 609C-T polymorphism have an increased risk of benzene hematotoxicity.

Long Name

NAD(P)H Dehydrogenase, Quinone 1

Alternate Names

Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, NQO1, QR1, Quinone Reductase 1

Entrez Gene IDs

1728 (Human); 18104 (Mouse)

Gene Symbol

NQO1

UniProt

NP_000894 (Human)

Additional NQO-1 Products

Product Documents for NQO-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NQO-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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