NPM1 [p Thr199] Antibody (SR1550) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21600

Recombinant Monoclonal Antibody
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NBP3-21600-100ul
NBP3-21600-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1550 expressed in HEK293

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NPM1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-NPM1 [Thr199] (Uniprot #: P06748)

Modification

p Thr 199

Specificity

Detects endogenous levels of total phospho-NPM1 [Thr199]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NPM1 [p Thr199] Antibody (SR1550) - BSA Free

Western Blot: NPM1 [p Thr 199] Antibody (SR1550) [NBP3-21600] -

Western Blot: NPM1 [p Thr 199] Antibody (SR1550) [NBP3-21600] - Western blot analysis using NBP3-21600 in (1) HeLa cell lysate; (2) HeLa cell treated with CA lysate.

Applications for NPM1 [p Thr199] Antibody (SR1550) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NPM1

Nucleophosmin (NPM) is a multi-functional protein that is involved in many cellular activities including cell growth, proliferation and transformation. It is frequently overexpressed in solid tumors, and has recently been found to be mutated and aberrantly localized in the cytoplasm of leukaemic blasts in patients with acute myeloid leukemia (AML).

NMP antibodies are useful tools as tumor markers in cancer research.

Long Name

Nucleophosmin/Nucleolar Phosphoprotein B23

Alternate Names

B23, Nucleophosmin, Numatrin

Gene Symbol

NPM1

Additional NPM1 Products

Product Documents for NPM1 [p Thr199] Antibody (SR1550) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NPM1 [p Thr199] Antibody (SR1550) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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