Noxa Antibody (114C307.1R) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07012
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Noxa Antibody (114C307.1R) - BSA Free [NBP3-07012]](https://resources.bio-techne.com/images/products/Noxa-Antibody-114C307-1R-Immunocytochemistry-Immunofluorescence-NBP3-07012-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Noxa Antibody (114C307.1R) - BSA Free [NBP3-07012]")
![Western Blot: Noxa Antibody (114C307.1R)BSA Free [NBP3-07012]](https://resources.bio-techne.com/images/products/Noxa-Antibody-114C307-1R-Western-Blot-NBP3-07012-img0003.jpg "Western Blot: Noxa Antibody (114C307.1R)BSA Free [NBP3-07012]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Knockout Validated, Immunohistochemistry, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence
Cited:
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 114C307.1R
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This Noxa antibody was developed by immunizing mice with a fusion protein containing human Noxa.
Reactivity Notes
Reactivity in rat is reported in scientific literature (Seda et al Hepatol Res. 2010, 40: 701-710).
Localization
Mitochondrion, also reported in Cytosol (PMID 21145489) & Nucleus (PMID 12879012).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Noxa Antibody (114C307.1R) - BSA Free
![Immunocytochemistry/ Immunofluorescence: Noxa Antibody (114C307.1R) - BSA Free [NBP3-07012]](https://resources.bio-techne.com/images/products/Noxa-Antibody-114C307-1R-Immunocytochemistry-Immunofluorescence-NBP3-07012-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Noxa Antibody (114C307.1R) - BSA Free [NBP3-07012]")
Immunocytochemistry/ Immunofluorescence: Noxa Antibody (114C307.1R) - BSA Free [NBP3-07012]
Immunocytochemistry/Immunofluorescence: Noxa Antibody (114C307.1R) [NBP3-07012] - ICC/IF analysis using the azide-free version of NB600-1159 at 1:10. HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.![Flow Cytometry: Noxa Antibody (114C307.1R) - BSA Free [NBP3-07012]](https://resources.bio-techne.com/images/products/Noxa-Antibody-114C307-1R-Flow-Cytometry-NBP3-07012-img0002.jpg "Flow Cytometry: Noxa Antibody (114C307.1R) - BSA Free [NBP3-07012]")
Flow Cytometry: Noxa Antibody (114C307.1R) - BSA Free [NBP3-07012]
Flow Cytometry: Noxa Antibody (114C307.1R) [NBP3-07012] - An intracellular stain was performed on Ntera2 cells with Noxa Antibody [114C307.1R] NBP3-07012 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).
Applications for Noxa Antibody (114C307.1R) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-5 ug/ml
Immunohistochemistry
1:200
Knockout Validated
reported in scientific literature (PMID: 32094511)
Western Blot
1 - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Noxa
Noxa mediated apoptosis may follow its transcriptional activation by p53 as part of the DNA damage response. However, Noxa expression may be induced by HIF-1 alpha under hypoxia, promoting apoptosis independently of p53 (1). Inhibition of the anti-apoptotic Bcl2 family member Myeloid cell leukemia-1 (Mcl-1) by Noxa, leads to the activation of pro-apoptotic Bcl2 homologous antagonist killer (Bak) and Bcl2 associated X (Bax) proteins, and apoptosis through the intrinsic mitochondrial pathway (2). Other BH3 only proteins such as Bim, Puma, Bad and Bid act via the same mechanism, targeting Mcl-1 and inducing Bak/Bax mediated mitochondrial outer membrane permeabilization (MOMP), cytochrome c release and caspase 9 activation (3). Overexpression of antiapoptotic Bcl2 family members is common in various types of cancer including prostate cancer. A recent study identified antiapoptotic Bcl2 proteins involved in the development of resistance towards the androgen receptor antagonist enzalutamide, which is used for the treatment of metastatic castration-resistant prostate cancer (mCRPC) (3).
References
1.Ploner, C., Kofler, R., & Villunger, A. (2008). Noxa: At the tip of the balance between life and death. Oncogene. https://doi.org/10.1038/onc.2009.46
2.Xiang, W., Yang, C. Y., & Bai, L. (2018). MCL-1 inhibition in cancer treatment. OncoTargets and Therapy. https://doi.org/10.2147/OTT.S146228
3.Pilling, A. B., & Hwang, C. (2019). Targeting prosurvival BCL2 signaling through Akt blockade sensitizes castration-resistant prostate cancer cells to enzalutamide. Prostate. https://doi.org/10.1002/pros.23843
Alternate Names
adult T cell leukemia-derived PMA-responsive, APR, Immediate-early-response protein APR, NOXAPMA-induced protein 1, phorbol-12-myristate-13-acetate-induced protein 1, PMA-Induced Protein 1, Protein Noxa
Gene Symbol
PMAIP1
Additional Noxa Products
Product Documents for Noxa Antibody (114C307.1R) - BSA Free
Product Specific Notices for Noxa Antibody (114C307.1R) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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