Notch-2 Antibody (PSH0-54)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32657

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32657
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH0-54

Concentration

1 mg/ml

View all Notch-2 Antibodies »

Product Specifications

Immunogen

Recombinant protein within human Notch-2 aa 2,151-2,350/2,471. (Uniprot: Q04721)

Localization

Cell membrane, Nucleus, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

265 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Notch-2 Antibody (PSH0-54)

Notch-2 Antibody (PSH0-54)

Western Blot: Notch-2 Antibody (PSH0-54) [NBP3-32657]

Western Blot: Notch-2 Antibody (PSH0-54) [NBP3-32657] - Western blot analysis of Notch-2 on different lysates with Rabbit anti-Notch-2 antibody (NBP3-32657) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PC-3M cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: HCT116 cell lysate
Lane 6: SW620 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 265 kDa
Observed band size: 110 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32657) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Notch-2 Antibody (PSH0-54)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Notch-2

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membraneFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Alternate Names

Notch2

Gene Symbol

NOTCH2

Additional Notch-2 Products

Product Documents for Notch-2 Antibody (PSH0-54)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Notch-2 Antibody (PSH0-54)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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