NOP58 Antibody

Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-46846]

Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-46846] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NOP58 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: NOP58 Antibody [NBP1-46846]

Immunoprecipitation: NOP58 Antibody [NBP1-46846] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NOP58 antibody NBP1-46846 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. NOP58 was also immunoprecipitated by rabbit anti-NOP58 antibody NBP1-46845, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Western Blot: NOP58 Antibody [NBP1-46846] -

NOP58 was stabilized by MIR4435-2HG and mediated the pro-tumorigenetic role of MIR4435-2HG in HCC. A The expression of NOP58 mRNA in HCC cells with MIR4435-2HG interference in HCC cells. B The expression of NOP58 protein in HCC cells with MIR4435-2HG overexpressed in HCC cells. C Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of CHX. D Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of MG132. E Expression analysis of NOP58 protein in HCC based on CPTAC database. F Representative images of the expression of NOP58 protein in paired tumor and nontumor tissues by IHC. G The relationship between MIR4435-2HG expression and NOP58 expression in 20 HCC samples. H Knockdown of NOP58 by siRNA in HCC cells. I Cellular proliferation of HCC cells was detected by CCK8 assay. J Cellular proliferation of HCC cells was detected by EdU assay. K Colony formation ability of HCC cells was detected. L The spheroids form ability of HCC cells. M Expression of stem cell markers were detected in the indicated groups by qPCR. The data are shown as mean +/- SD. *P < 0.05, **P < 0.01, ***P < 0.001. White scale bar, 100 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37891494), licensed under a CC-BY license. Not internally tested by Novus Biologicals.