NM23-H1 Antibody (23GB2195)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51024
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB2195
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human NM23-H1 (Uniprot: P15531)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NM23-H1 Antibody (23GB2195)
Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (23GB2195)[NBP3-51024] -
NM23-H1 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: NME1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;NME1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (23GB2195)[NBP3-51024] -
Immunocytochemical staining of HepG2 cells with NM23-H1 antibody (1:1,000). Nuclei were stained blue with DAPI; NM23-H1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
Flow Cytometry: NM23-H1 Antibody (23GB2195)[NBP3-51024] -
Validation of NM23-H1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NM23-H1 antibody (1:2,000) and analyzed using BD flow cytometer.
Applications for NM23-H1 Antibody (23GB2195)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NM23-H1
Long Name
Non-metastatic Protein 23 Homolog 1
Alternate Names
GAAD, NDKA, NDPKA, NM23A, NM23H1, NME1
Gene Symbol
NME1
Additional NM23-H1 Products
Product Documents for NM23-H1 Antibody (23GB2195)
Product Specific Notices for NM23-H1 Antibody (23GB2195)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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