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NLRP1/NALP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54899

Novus Biologicals, part of Bio-Techne
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NBP1-54899

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to NLRP1(NLR family, pyrin domain containing 1) The peptide sequence was selected from the N terminal of NLRP1 (NP_001028225). DTQEPRIVILQGAAGIGKSTLARQVKEAWGRGQLYGDRFQHVFYFSCREL. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30342528)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for NLRP1/NALP1 Antibody - BSA Free

Western Blot: NLRP1/NALP1 Antibody [NBP1-54899]

Western Blot: NLRP1/NALP1 Antibody [NBP1-54899]

Western Blot: NLRP1/NALP1 Antibody [NBP1-54899] - NCI-H226 cell lysate, concentration 0.2-1 ug/ml.
NLRP1/NALP1 Antibody

Western Blot: NLRP1/NALP1 Antibody [NBP1-54899] -

Western Blot: NLRP1/NALP1 Antibody [NBP1-54899] Human MCF7 whole cell. Antibody Dilution: 1ug/ml
NLRP1/NALP1 Antibody - BSA Free

Western Blot: NLRP1/NALP1 Antibody - BSA Free [NBP1-54899] -

RTP801 silencing in the R6/1 mouse hippocampal neurons reduces the levels of the inflammasome components. (a) Immunoblottings for NLRP1, cleaved NLRP1, procaspase 1, cleaved caspase 1, ASC/TMS1, and GFP as loading control for transduced neurons in the dorsal hippocampus of 4.5-month-old WT shCt, WT shRTP801, R6/1 shCt, and R6/1 shRTP801 mice. (b–f) Densitometric quantification of NRLP1 (treatment effect: F(1, 26) = 24.89, p < 0.0001; interaction: F(1, 26) = 1.175, p = 0.2883), cleaved NLRP1 (treatment effect: F(1, 29) = 44.18, p < 0.0001; genotype effect: F(1, 29) = 15.32, p = 0.0005; interaction: F(1, 29) = 6.588, P = 0.0157), ASC/TMS1 (treatment effect: F(1, 31) = 40.72, p < 0.0001; genotype effect: F(1, 31) = 17.85, p = 0.0002; interaction: F(1, 31) = 15.91, p = 0.0004), procaspase 1 (treatment effect: F(1, 30) = 26.06, p < 0.0001; interaction: F(1, 30) = 10.82, p = 0.0026) and cleaved caspase 1 (treatment effect: F(1, 30) = 25.57, p < 0.0001; interaction: F(1, 30) = 5.551, p = 0.0252) as in (a) for the hippocampus. Densitometric quantification of all proteins is expressed as the mean +/- SEM. All data were analyzed by two-way ANOVA followed by Bonferroni’s post hoc test. * p < 0.05, ** p < 0.01, and *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35053183), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for NLRP1/NALP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1.0 ug/ml
Application Notes
Use in Immunocytochemistry/immunofluorescence and Immunohistochemistry-Frozen reported in scientific literature (PMID 24008734).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NLRP1/NALP1

This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like

Long Name

NLR Family, Pyrin Domain Containing 1

Alternate Names

CARD7, CLR17.1, DEFCAP, NALP1, PP1044, SLEV1, VAMAS1

Entrez Gene IDs

22861 (Human)

Gene Symbol

NLRP1

UniProt

Additional NLRP1/NALP1 Products

Product Documents for NLRP1/NALP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NLRP1/NALP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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